Gas Chromatography
Chromatography: “Chroma” is Greek meaning “colour” and “graphein” is Greek meaning “to write”
Definition: To separate a mixture by relative attraction of the components as a mobile phase (a liquid or gas) passes over the stationary phase (a solid or liquid).
Mobile phase: a liquid or gas which moves through the column/tubing
Stationary phase: is a solid or liquid which is part on the inside of the column or tubing the unknown solution is being passed through, this material is stationary/doesn’t move
Definition: To separate a mixture by relative attraction of the components as a mobile phase (a liquid or gas) passes over the stationary phase (a solid or liquid).
Mobile phase: a liquid or gas which moves through the column/tubing
Stationary phase: is a solid or liquid which is part on the inside of the column or tubing the unknown solution is being passed through, this material is stationary/doesn’t move
Gas Chromatograph (GC):
Procedure:
1) Check carrier gas: open main valve on the carrier gas cylinder-make sure the gas is being supplied evenly.
2) Check for leaks in the tubing system
3) Start up the GS following steps in the procedure manual such as:
a. Inject sample (which is vaporized) into the head of the chromatograph column.
b. The sample is transported via the inert gas (mobile phase).
c. The column contains a liquid stationary phase-which absorbs unto the surface of an inert solid.
d. Detector: is a Flame Ionization Detector
Side note: Most columns are 1.5-10m in length and have an internal diameter of 2-4mm. (Cited from teaching.shu.ac.uk/hwb/chemistry/tutorials/chrom/gaschrm.htm
4) Print results and perform proper shut-down procedures, see User’s Manual
5) Close main valve of carrier gas cylinder
Procedure:
1) Check carrier gas: open main valve on the carrier gas cylinder-make sure the gas is being supplied evenly.
2) Check for leaks in the tubing system
3) Start up the GS following steps in the procedure manual such as:
a. Inject sample (which is vaporized) into the head of the chromatograph column.
b. The sample is transported via the inert gas (mobile phase).
c. The column contains a liquid stationary phase-which absorbs unto the surface of an inert solid.
d. Detector: is a Flame Ionization Detector
Side note: Most columns are 1.5-10m in length and have an internal diameter of 2-4mm. (Cited from teaching.shu.ac.uk/hwb/chemistry/tutorials/chrom/gaschrm.htm
4) Print results and perform proper shut-down procedures, see User’s Manual
5) Close main valve of carrier gas cylinder